Staining:Article Title: Variant-proof high affinity ACE2 antagonist limits SARS-CoV-2 replication in upper and lower airways
Article Snippet: .. Briefly, cryopreserved PBMC were thawed and stained with the following antibodies (monoclonal unless indicated): (1) IgD FITC, goat polyclonal antibody, Southern Biotech #2030-02—Lot #A2118-WF09C at a dilution of 1:40; (2) IgM PerCP-Cy5.5, clone G20-127, BD Biosciences #561285—Lot #0307134 at 1:40; (3) IgA Dy405, goat polyclonal antibody, Jackson ImmunoResearch #109-475-011—Lot #155196 at 1:40; (4) CD20 BV570, clone 2H7, Biolegend #302332—Lot #B301458 at 1:40; (5) CD27 BV650, clone O323, Biolegend #302828—Lot #B273921 at 1:20; (6) CD14 BV785, clone M5E2, Biolegend #301840—Lot #B327948 at 1:80; (7) CD16 BUV496, clone 3G8, BD Biosciences #564653—Lot #0288806 at 1:40; (8) CD4 BUV737, clone SK3, BD Biosciences #564305—Lot #0282762 at 1:40; (9) CD8 BUV395, clone RPA-T8, BD Biosciences #563795—Lot #9346411 at 1:80; (10) CD19 APC, clone J3-119, Beckman Coulter #IM2470U—Lot #200092 at 1:20; (11) IgG Alexa 700, clone G18-145, BD Biosciences #561296—Lot #0135021 at 1:20; (12) CD3 APC-Cy7, clone SP34.2, BD Biosciences #557757—Lot #0223215 at 1:40; (13) CD38 PE, clone OKT10, Caprico Biotech #100826—Lot #8AE4 at 1:640; (14) CD21 PE-Cy5, clone B-ly4, BD Biosciences #551064—Lot #0072939 at 1:20; and (15) CXCR5 PE-Cy7, clone MU5UBEE, Thermo Fisher #25-9185-42—Lot #2312036 at 1:40. .. Stained cells were then incubated with streptavidin-BV605 (BD Biosciences) labeled Delta S-2P, BA1 S-2P or RBD-62 and streptavidin-BUV661 (BD Biosciences) labeled WA1 or Delta S-2P for 30 min at 4 °C (protected from light).
Recombinase Polymerase Amplification:Article Title: Variant-proof high affinity ACE2 antagonist limits SARS-CoV-2 replication in upper and lower airways
Article Snippet: .. Briefly, cryopreserved PBMC were thawed and stained with the following antibodies (monoclonal unless indicated): (1) IgD FITC, goat polyclonal antibody, Southern Biotech #2030-02—Lot #A2118-WF09C at a dilution of 1:40; (2) IgM PerCP-Cy5.5, clone G20-127, BD Biosciences #561285—Lot #0307134 at 1:40; (3) IgA Dy405, goat polyclonal antibody, Jackson ImmunoResearch #109-475-011—Lot #155196 at 1:40; (4) CD20 BV570, clone 2H7, Biolegend #302332—Lot #B301458 at 1:40; (5) CD27 BV650, clone O323, Biolegend #302828—Lot #B273921 at 1:20; (6) CD14 BV785, clone M5E2, Biolegend #301840—Lot #B327948 at 1:80; (7) CD16 BUV496, clone 3G8, BD Biosciences #564653—Lot #0288806 at 1:40; (8) CD4 BUV737, clone SK3, BD Biosciences #564305—Lot #0282762 at 1:40; (9) CD8 BUV395, clone RPA-T8, BD Biosciences #563795—Lot #9346411 at 1:80; (10) CD19 APC, clone J3-119, Beckman Coulter #IM2470U—Lot #200092 at 1:20; (11) IgG Alexa 700, clone G18-145, BD Biosciences #561296—Lot #0135021 at 1:20; (12) CD3 APC-Cy7, clone SP34.2, BD Biosciences #557757—Lot #0223215 at 1:40; (13) CD38 PE, clone OKT10, Caprico Biotech #100826—Lot #8AE4 at 1:640; (14) CD21 PE-Cy5, clone B-ly4, BD Biosciences #551064—Lot #0072939 at 1:20; and (15) CXCR5 PE-Cy7, clone MU5UBEE, Thermo Fisher #25-9185-42—Lot #2312036 at 1:40. .. Stained cells were then incubated with streptavidin-BV605 (BD Biosciences) labeled Delta S-2P, BA1 S-2P or RBD-62 and streptavidin-BUV661 (BD Biosciences) labeled WA1 or Delta S-2P for 30 min at 4 °C (protected from light).
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